John J. Ryan, Wei Zhao, Gregorio Gomez, et.al., The Journal of Immunology, 2007, 179:665-672, DOI:10.4049/jimmunol.179.1.65
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Fullerene are a class of novel carbon allotropes that may have practical applications in biotechnology and medicine. { Fullerene nanomaterials inhibit the allergic response }. Human mast cells (MC) and peripheral blood basophils are critical cells involved in the initiation and propagation of several inflammatory conditions, mainly type I hypersensitivity. We report an unanticipated role of Fullerene as a negative regulator of allergic mediator release that suppresses Ag-driven type I hypersensitivity. Human MC and peripheral blood basophils exhibited a significant inhibition of IgE dependent mediator release when preincubated with C60 fullerene . Protein microarray demonstrated that inhibition of mediator release involves profound reductions in the activation of signaling molecules involved in mediator release and oxidative stress. { Fullerene nanomaterials inhibit the allergic response }. Follow-up studies demonstrated that the tyrosine phosphorylation of Syk was dramatically inhibited in Ag-challenged cells first incubated with fullerenes. In addition, fullerene preincubation significantly inhibited IgE-induced elevation in cytoplasmic reactive oxygen species levels. { Fullerene nanomaterials inhibit the allergic response }. Furthermore, fullerenes prevented the in vivo release of histamine and drop in core body temperature in vivo using a MC-dependent model of anaphylaxis. These findings identify a new biological function for fullerenes and may represent a novel way to control MC-dependent diseases including asthma, inflammatory arthritis, heart disease, and multiple sclerosis. The Journal of Immunology, 2007, 179: 665–672.